Type a model, reaction, metabolite, or gene (BiGG ID or name) into the search box. If you want to search within a specific model, select it before submitting.

After you search, results appear in four sections:

• Models — model-level coverage, reaction quality classes, Δ_rG° distributions, and compartment settings.
• Reactions — Δ_rG° values, equations, and variability across models (SD).
• Metabolites — IDs, names, formulas, structures (if available), and related reactions.
• Genes — linked models and reactions, plus GPR mappings.

To view detailed Δ_rG° information for a reaction, click Show Δ_rG°.

• Reaction Classification Chart — shows how reactions are categorized: boundary, unstructured, unbalanced, and covered (covered is split into reversible and irreversible).
• Reaction Class Definitions — short explanations for each category.
• Δ_rG° Distribution Chart — a histogram of Δ_rG° with a reference line at Δ_rG° = 0.
• Compartment Conditions — pH, T, I, pMg, and E used when calculating Δ_rG° for this model.
• Use the download option to export model-level results.

For reactions, you will see BiGG IDs, names, Δ_rG° values, SD, and reaction equations, including how the same reaction appears across different models.

For metabolites, you will see BiGG IDs, chemical formulas, structures (if available), and the reactions in which each metabolite participates.

For genes, you will see gene IDs, the models that contain each gene, linked reactions, and the corresponding GPR information.

Choose the identifier type used in your equation: SMILES, InChI, InChI Key, KEGG, MetaNetX, HMDB, Name, BiGG (universal), ChEBI, PubChem, or Mixed.

✔ For best accuracy, use SMILES or InChI whenever possible.

If you use BiGG metabolite IDs, remove compartment suffixes (e.g., succ_c → succ).

• Put spaces around + and = (example: A + B = C).
• Put a space between a coefficient and a compound (example: 2 H2O).
• If you mix identifier types, use the format TYPE:ID.

You can load example equations using the auto-fill options on the prediction page.

For a single reaction, the results page shows:

• The predicted Δ_rG° (kJ/mol).
• The standard deviation (SD).
• The reaction equation you submitted.
• The conditions used (pH, I, pMg, E, T).

In batch mode, enter one equation per line. All reactions use the same identifier type and the same global settings for pH, I, pMg, and E. Temperature is fixed at 298.15 K.

Batch Output

• A table with Δ_rG° and SD for each reaction.
• Failed entries are marked with an error message.
• You can download the results as a CSV file.